Table 5

Sequences of oligonucleotide primers for PCR amplification of IL-12RB1 coding exons

		Length
Covering exons	Primer sequence(forward and reverse (5′-3′))	Primer	Product	Tm	CG%	Accession number
Exon:1,2,3,4,5,6,7,8	TCGCAGGTGGCAGAGAGG	18	845	61.39	66.67	XM_011527977
Exon:1,2,3,4,5,6,7,8	GCTGGGTTGGCTGCTCTTT	19	845	60.91	57.89	XM_011527977
Exon:6,7,8,9,10,11	CGGACACCCAGCAGCCCA	18	801	64.71	72.22	XM_011527976
Exon:6,7,8,9,10,11	CAGGACCGTAGACCACAAG	19	801	57.19	57.89	XM_011527976
Exon:10,11,12,13,14,15,16,17	CATTGAATGGCAGCCTGTG	19	903	57.27	52.63	XM_011527975
Exon:10,11,12,13,14,15,16,17	GAGTCACTCACCCTCTCTG	19	903	56.18	57.89	XM_011527975